intensity-based image correlation in Search Results


imaris intensity based colocalization analysis  (Oxford Instruments)
99
Oxford Instruments imaris intensity based colocalization analysis
( A ) High-magnification images of pachytene nuclei, oocyte, and sperm from a young adult hermaphrodite, demonstrating HIS-72::mCherry (H3.3) and HIS-55::GFP (H3). White arrowheads indicate chromatin regions showing presence of H3 but absence of H3.3. ( B ) Immunofluorescence (confocal) of HIS-72::mCherry (red), HIS-55::GFP (green), H3K27me2/3 (blue), and H3K36me2 (magenta) in pachytene nuclei. White arrowheads indicate chromosome X identified by the presence of H3 and H3K27me2/3 but absence of H3.3 and H3K36me2. ( C ) Immunofluorescence (Airyscan) of HIS-72::mCherry (red), HIS-55::Ollas (green), and H3K27me2/3 (magenta; top) and H3K36me2 (magenta; bottom) in pachytene nuclei. White arrowheads indicate distinct chromatin domains marked by the <t>colocalization</t> of H3K27me2/3 or H3K36me2 to the presence of H3 or H3.3, respectively. ( D ) Colocalization analysis of Airyscan immunofluorescent micrographs of H3K27me2/3 or H3K36me2 with H3::Ollas and H3.3::mCherry. All quantifications = average ± SE. Unpaired t test; **** P ≤ 0.0001 and ** P ≤ 0.01. Scale bars, 5 μm.
Imaris Intensity Based Colocalization Analysis, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/imaris intensity based colocalization analysis/product/Oxford Instruments
Average 99 stars, based on 1 article reviews
imaris intensity based colocalization analysis - by Bioz Stars, 2026-06
99/100 stars
  Buy from Supplier
intensity-based thresholding  (MetaMorph Inc)
90
MetaMorph Inc intensity-based thresholding
( A ) High-magnification images of pachytene nuclei, oocyte, and sperm from a young adult hermaphrodite, demonstrating HIS-72::mCherry (H3.3) and HIS-55::GFP (H3). White arrowheads indicate chromatin regions showing presence of H3 but absence of H3.3. ( B ) Immunofluorescence (confocal) of HIS-72::mCherry (red), HIS-55::GFP (green), H3K27me2/3 (blue), and H3K36me2 (magenta) in pachytene nuclei. White arrowheads indicate chromosome X identified by the presence of H3 and H3K27me2/3 but absence of H3.3 and H3K36me2. ( C ) Immunofluorescence (Airyscan) of HIS-72::mCherry (red), HIS-55::Ollas (green), and H3K27me2/3 (magenta; top) and H3K36me2 (magenta; bottom) in pachytene nuclei. White arrowheads indicate distinct chromatin domains marked by the <t>colocalization</t> of H3K27me2/3 or H3K36me2 to the presence of H3 or H3.3, respectively. ( D ) Colocalization analysis of Airyscan immunofluorescent micrographs of H3K27me2/3 or H3K36me2 with H3::Ollas and H3.3::mCherry. All quantifications = average ± SE. Unpaired t test; **** P ≤ 0.0001 and ** P ≤ 0.01. Scale bars, 5 μm.
Intensity Based Thresholding, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/intensity-based thresholding/product/MetaMorph Inc
Average 90 stars, based on 1 article reviews
intensity-based thresholding - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
intensity-based registration  (NeuroInterventions Inc)
90
NeuroInterventions Inc intensity-based registration
( A ) High-magnification images of pachytene nuclei, oocyte, and sperm from a young adult hermaphrodite, demonstrating HIS-72::mCherry (H3.3) and HIS-55::GFP (H3). White arrowheads indicate chromatin regions showing presence of H3 but absence of H3.3. ( B ) Immunofluorescence (confocal) of HIS-72::mCherry (red), HIS-55::GFP (green), H3K27me2/3 (blue), and H3K36me2 (magenta) in pachytene nuclei. White arrowheads indicate chromosome X identified by the presence of H3 and H3K27me2/3 but absence of H3.3 and H3K36me2. ( C ) Immunofluorescence (Airyscan) of HIS-72::mCherry (red), HIS-55::Ollas (green), and H3K27me2/3 (magenta; top) and H3K36me2 (magenta; bottom) in pachytene nuclei. White arrowheads indicate distinct chromatin domains marked by the <t>colocalization</t> of H3K27me2/3 or H3K36me2 to the presence of H3 or H3.3, respectively. ( D ) Colocalization analysis of Airyscan immunofluorescent micrographs of H3K27me2/3 or H3K36me2 with H3::Ollas and H3.3::mCherry. All quantifications = average ± SE. Unpaired t test; **** P ≤ 0.0001 and ** P ≤ 0.01. Scale bars, 5 μm.
Intensity Based Registration, supplied by NeuroInterventions Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/intensity-based registration/product/NeuroInterventions Inc
Average 90 stars, based on 1 article reviews
intensity-based registration - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
pmt h10770b-40  (Hamamatsu)
90
Hamamatsu pmt h10770b-40
( A ) High-magnification images of pachytene nuclei, oocyte, and sperm from a young adult hermaphrodite, demonstrating HIS-72::mCherry (H3.3) and HIS-55::GFP (H3). White arrowheads indicate chromatin regions showing presence of H3 but absence of H3.3. ( B ) Immunofluorescence (confocal) of HIS-72::mCherry (red), HIS-55::GFP (green), H3K27me2/3 (blue), and H3K36me2 (magenta) in pachytene nuclei. White arrowheads indicate chromosome X identified by the presence of H3 and H3K27me2/3 but absence of H3.3 and H3K36me2. ( C ) Immunofluorescence (Airyscan) of HIS-72::mCherry (red), HIS-55::Ollas (green), and H3K27me2/3 (magenta; top) and H3K36me2 (magenta; bottom) in pachytene nuclei. White arrowheads indicate distinct chromatin domains marked by the <t>colocalization</t> of H3K27me2/3 or H3K36me2 to the presence of H3 or H3.3, respectively. ( D ) Colocalization analysis of Airyscan immunofluorescent micrographs of H3K27me2/3 or H3K36me2 with H3::Ollas and H3.3::mCherry. All quantifications = average ± SE. Unpaired t test; **** P ≤ 0.0001 and ** P ≤ 0.01. Scale bars, 5 μm.
Pmt H10770b 40, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pmt h10770b-40/product/Hamamatsu
Average 90 stars, based on 1 article reviews
pmt h10770b-40 - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
intensity-based segmentation algorithm  (MIMVista corp)
90
MIMVista corp intensity-based segmentation algorithm
( A ) High-magnification images of pachytene nuclei, oocyte, and sperm from a young adult hermaphrodite, demonstrating HIS-72::mCherry (H3.3) and HIS-55::GFP (H3). White arrowheads indicate chromatin regions showing presence of H3 but absence of H3.3. ( B ) Immunofluorescence (confocal) of HIS-72::mCherry (red), HIS-55::GFP (green), H3K27me2/3 (blue), and H3K36me2 (magenta) in pachytene nuclei. White arrowheads indicate chromosome X identified by the presence of H3 and H3K27me2/3 but absence of H3.3 and H3K36me2. ( C ) Immunofluorescence (Airyscan) of HIS-72::mCherry (red), HIS-55::Ollas (green), and H3K27me2/3 (magenta; top) and H3K36me2 (magenta; bottom) in pachytene nuclei. White arrowheads indicate distinct chromatin domains marked by the <t>colocalization</t> of H3K27me2/3 or H3K36me2 to the presence of H3 or H3.3, respectively. ( D ) Colocalization analysis of Airyscan immunofluorescent micrographs of H3K27me2/3 or H3K36me2 with H3::Ollas and H3.3::mCherry. All quantifications = average ± SE. Unpaired t test; **** P ≤ 0.0001 and ** P ≤ 0.01. Scale bars, 5 μm.
Intensity Based Segmentation Algorithm, supplied by MIMVista corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/intensity-based segmentation algorithm/product/MIMVista corp
Average 90 stars, based on 1 article reviews
intensity-based segmentation algorithm - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
spectralis oct  (heidelberg engineering)
90
heidelberg engineering spectralis oct
( A ) High-magnification images of pachytene nuclei, oocyte, and sperm from a young adult hermaphrodite, demonstrating HIS-72::mCherry (H3.3) and HIS-55::GFP (H3). White arrowheads indicate chromatin regions showing presence of H3 but absence of H3.3. ( B ) Immunofluorescence (confocal) of HIS-72::mCherry (red), HIS-55::GFP (green), H3K27me2/3 (blue), and H3K36me2 (magenta) in pachytene nuclei. White arrowheads indicate chromosome X identified by the presence of H3 and H3K27me2/3 but absence of H3.3 and H3K36me2. ( C ) Immunofluorescence (Airyscan) of HIS-72::mCherry (red), HIS-55::Ollas (green), and H3K27me2/3 (magenta; top) and H3K36me2 (magenta; bottom) in pachytene nuclei. White arrowheads indicate distinct chromatin domains marked by the <t>colocalization</t> of H3K27me2/3 or H3K36me2 to the presence of H3 or H3.3, respectively. ( D ) Colocalization analysis of Airyscan immunofluorescent micrographs of H3K27me2/3 or H3K36me2 with H3::Ollas and H3.3::mCherry. All quantifications = average ± SE. Unpaired t test; **** P ≤ 0.0001 and ** P ≤ 0.01. Scale bars, 5 μm.
Spectralis Oct, supplied by heidelberg engineering, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/spectralis oct/product/heidelberg engineering
Average 90 stars, based on 1 article reviews
spectralis oct - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
intensity-based free form deformation algorithm mim 5.4  (MIM Software Inc)
90
MIM Software Inc intensity-based free form deformation algorithm mim 5.4
( A ) High-magnification images of pachytene nuclei, oocyte, and sperm from a young adult hermaphrodite, demonstrating HIS-72::mCherry (H3.3) and HIS-55::GFP (H3). White arrowheads indicate chromatin regions showing presence of H3 but absence of H3.3. ( B ) Immunofluorescence (confocal) of HIS-72::mCherry (red), HIS-55::GFP (green), H3K27me2/3 (blue), and H3K36me2 (magenta) in pachytene nuclei. White arrowheads indicate chromosome X identified by the presence of H3 and H3K27me2/3 but absence of H3.3 and H3K36me2. ( C ) Immunofluorescence (Airyscan) of HIS-72::mCherry (red), HIS-55::Ollas (green), and H3K27me2/3 (magenta; top) and H3K36me2 (magenta; bottom) in pachytene nuclei. White arrowheads indicate distinct chromatin domains marked by the <t>colocalization</t> of H3K27me2/3 or H3K36me2 to the presence of H3 or H3.3, respectively. ( D ) Colocalization analysis of Airyscan immunofluorescent micrographs of H3K27me2/3 or H3K36me2 with H3::Ollas and H3.3::mCherry. All quantifications = average ± SE. Unpaired t test; **** P ≤ 0.0001 and ** P ≤ 0.01. Scale bars, 5 μm.
Intensity Based Free Form Deformation Algorithm Mim 5.4, supplied by MIM Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/intensity-based free form deformation algorithm mim 5.4/product/MIM Software Inc
Average 90 stars, based on 1 article reviews
intensity-based free form deformation algorithm mim 5.4 - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
fluo-8®, am  (AAT Bioquest)
90
AAT Bioquest fluo-8®, am
( A ) High-magnification images of pachytene nuclei, oocyte, and sperm from a young adult hermaphrodite, demonstrating HIS-72::mCherry (H3.3) and HIS-55::GFP (H3). White arrowheads indicate chromatin regions showing presence of H3 but absence of H3.3. ( B ) Immunofluorescence (confocal) of HIS-72::mCherry (red), HIS-55::GFP (green), H3K27me2/3 (blue), and H3K36me2 (magenta) in pachytene nuclei. White arrowheads indicate chromosome X identified by the presence of H3 and H3K27me2/3 but absence of H3.3 and H3K36me2. ( C ) Immunofluorescence (Airyscan) of HIS-72::mCherry (red), HIS-55::Ollas (green), and H3K27me2/3 (magenta; top) and H3K36me2 (magenta; bottom) in pachytene nuclei. White arrowheads indicate distinct chromatin domains marked by the <t>colocalization</t> of H3K27me2/3 or H3K36me2 to the presence of H3 or H3.3, respectively. ( D ) Colocalization analysis of Airyscan immunofluorescent micrographs of H3K27me2/3 or H3K36me2 with H3::Ollas and H3.3::mCherry. All quantifications = average ± SE. Unpaired t test; **** P ≤ 0.0001 and ** P ≤ 0.01. Scale bars, 5 μm.
Fluo 8®, Am, supplied by AAT Bioquest, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fluo-8®, am/product/AAT Bioquest
Average 90 stars, based on 1 article reviews
fluo-8®, am - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
intensity based calcium indicator fluo-81, am  (AAT Bioquest)
90
AAT Bioquest intensity based calcium indicator fluo-81, am
( A ) High-magnification images of pachytene nuclei, oocyte, and sperm from a young adult hermaphrodite, demonstrating HIS-72::mCherry (H3.3) and HIS-55::GFP (H3). White arrowheads indicate chromatin regions showing presence of H3 but absence of H3.3. ( B ) Immunofluorescence (confocal) of HIS-72::mCherry (red), HIS-55::GFP (green), H3K27me2/3 (blue), and H3K36me2 (magenta) in pachytene nuclei. White arrowheads indicate chromosome X identified by the presence of H3 and H3K27me2/3 but absence of H3.3 and H3K36me2. ( C ) Immunofluorescence (Airyscan) of HIS-72::mCherry (red), HIS-55::Ollas (green), and H3K27me2/3 (magenta; top) and H3K36me2 (magenta; bottom) in pachytene nuclei. White arrowheads indicate distinct chromatin domains marked by the <t>colocalization</t> of H3K27me2/3 or H3K36me2 to the presence of H3 or H3.3, respectively. ( D ) Colocalization analysis of Airyscan immunofluorescent micrographs of H3K27me2/3 or H3K36me2 with H3::Ollas and H3.3::mCherry. All quantifications = average ± SE. Unpaired t test; **** P ≤ 0.0001 and ** P ≤ 0.01. Scale bars, 5 μm.
Intensity Based Calcium Indicator Fluo 81, Am, supplied by AAT Bioquest, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/intensity based calcium indicator fluo-81, am/product/AAT Bioquest
Average 90 stars, based on 1 article reviews
intensity based calcium indicator fluo-81, am - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
spectrofluorimeter quanta master 40 intensity based spectrofluorimeter  (HORIBA Ltd)
90
HORIBA Ltd spectrofluorimeter quanta master 40 intensity based spectrofluorimeter
( A ) High-magnification images of pachytene nuclei, oocyte, and sperm from a young adult hermaphrodite, demonstrating HIS-72::mCherry (H3.3) and HIS-55::GFP (H3). White arrowheads indicate chromatin regions showing presence of H3 but absence of H3.3. ( B ) Immunofluorescence (confocal) of HIS-72::mCherry (red), HIS-55::GFP (green), H3K27me2/3 (blue), and H3K36me2 (magenta) in pachytene nuclei. White arrowheads indicate chromosome X identified by the presence of H3 and H3K27me2/3 but absence of H3.3 and H3K36me2. ( C ) Immunofluorescence (Airyscan) of HIS-72::mCherry (red), HIS-55::Ollas (green), and H3K27me2/3 (magenta; top) and H3K36me2 (magenta; bottom) in pachytene nuclei. White arrowheads indicate distinct chromatin domains marked by the <t>colocalization</t> of H3K27me2/3 or H3K36me2 to the presence of H3 or H3.3, respectively. ( D ) Colocalization analysis of Airyscan immunofluorescent micrographs of H3K27me2/3 or H3K36me2 with H3::Ollas and H3.3::mCherry. All quantifications = average ± SE. Unpaired t test; **** P ≤ 0.0001 and ** P ≤ 0.01. Scale bars, 5 μm.
Spectrofluorimeter Quanta Master 40 Intensity Based Spectrofluorimeter, supplied by HORIBA Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/spectrofluorimeter quanta master 40 intensity based spectrofluorimeter/product/HORIBA Ltd
Average 90 stars, based on 1 article reviews
spectrofluorimeter quanta master 40 intensity based spectrofluorimeter - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
intensity based deformable image registration software mim-vista version 5.1  (MIM Software Inc)
90
MIM Software Inc intensity based deformable image registration software mim-vista version 5.1
( A ) High-magnification images of pachytene nuclei, oocyte, and sperm from a young adult hermaphrodite, demonstrating HIS-72::mCherry (H3.3) and HIS-55::GFP (H3). White arrowheads indicate chromatin regions showing presence of H3 but absence of H3.3. ( B ) Immunofluorescence (confocal) of HIS-72::mCherry (red), HIS-55::GFP (green), H3K27me2/3 (blue), and H3K36me2 (magenta) in pachytene nuclei. White arrowheads indicate chromosome X identified by the presence of H3 and H3K27me2/3 but absence of H3.3 and H3K36me2. ( C ) Immunofluorescence (Airyscan) of HIS-72::mCherry (red), HIS-55::Ollas (green), and H3K27me2/3 (magenta; top) and H3K36me2 (magenta; bottom) in pachytene nuclei. White arrowheads indicate distinct chromatin domains marked by the <t>colocalization</t> of H3K27me2/3 or H3K36me2 to the presence of H3 or H3.3, respectively. ( D ) Colocalization analysis of Airyscan immunofluorescent micrographs of H3K27me2/3 or H3K36me2 with H3::Ollas and H3.3::mCherry. All quantifications = average ± SE. Unpaired t test; **** P ≤ 0.0001 and ** P ≤ 0.01. Scale bars, 5 μm.
Intensity Based Deformable Image Registration Software Mim Vista Version 5.1, supplied by MIM Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/intensity based deformable image registration software mim-vista version 5.1/product/MIM Software Inc
Average 90 stars, based on 1 article reviews
intensity based deformable image registration software mim-vista version 5.1 - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
dapi intensity  (MetaMorph Inc)
90
MetaMorph Inc dapi intensity
( A ) High-magnification images of pachytene nuclei, oocyte, and sperm from a young adult hermaphrodite, demonstrating HIS-72::mCherry (H3.3) and HIS-55::GFP (H3). White arrowheads indicate chromatin regions showing presence of H3 but absence of H3.3. ( B ) Immunofluorescence (confocal) of HIS-72::mCherry (red), HIS-55::GFP (green), H3K27me2/3 (blue), and H3K36me2 (magenta) in pachytene nuclei. White arrowheads indicate chromosome X identified by the presence of H3 and H3K27me2/3 but absence of H3.3 and H3K36me2. ( C ) Immunofluorescence (Airyscan) of HIS-72::mCherry (red), HIS-55::Ollas (green), and H3K27me2/3 (magenta; top) and H3K36me2 (magenta; bottom) in pachytene nuclei. White arrowheads indicate distinct chromatin domains marked by the <t>colocalization</t> of H3K27me2/3 or H3K36me2 to the presence of H3 or H3.3, respectively. ( D ) Colocalization analysis of Airyscan immunofluorescent micrographs of H3K27me2/3 or H3K36me2 with H3::Ollas and H3.3::mCherry. All quantifications = average ± SE. Unpaired t test; **** P ≤ 0.0001 and ** P ≤ 0.01. Scale bars, 5 μm.
Dapi Intensity, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dapi intensity/product/MetaMorph Inc
Average 90 stars, based on 1 article reviews
dapi intensity - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier

Image Search Results


( A ) High-magnification images of pachytene nuclei, oocyte, and sperm from a young adult hermaphrodite, demonstrating HIS-72::mCherry (H3.3) and HIS-55::GFP (H3). White arrowheads indicate chromatin regions showing presence of H3 but absence of H3.3. ( B ) Immunofluorescence (confocal) of HIS-72::mCherry (red), HIS-55::GFP (green), H3K27me2/3 (blue), and H3K36me2 (magenta) in pachytene nuclei. White arrowheads indicate chromosome X identified by the presence of H3 and H3K27me2/3 but absence of H3.3 and H3K36me2. ( C ) Immunofluorescence (Airyscan) of HIS-72::mCherry (red), HIS-55::Ollas (green), and H3K27me2/3 (magenta; top) and H3K36me2 (magenta; bottom) in pachytene nuclei. White arrowheads indicate distinct chromatin domains marked by the colocalization of H3K27me2/3 or H3K36me2 to the presence of H3 or H3.3, respectively. ( D ) Colocalization analysis of Airyscan immunofluorescent micrographs of H3K27me2/3 or H3K36me2 with H3::Ollas and H3.3::mCherry. All quantifications = average ± SE. Unpaired t test; **** P ≤ 0.0001 and ** P ≤ 0.01. Scale bars, 5 μm.

Journal: Science Advances

Article Title: Developmentally programmed histone H3 expression regulates cellular plasticity at the parental-to-early embryo transition

doi: 10.1126/sciadv.adh0411

Figure Lengend Snippet: ( A ) High-magnification images of pachytene nuclei, oocyte, and sperm from a young adult hermaphrodite, demonstrating HIS-72::mCherry (H3.3) and HIS-55::GFP (H3). White arrowheads indicate chromatin regions showing presence of H3 but absence of H3.3. ( B ) Immunofluorescence (confocal) of HIS-72::mCherry (red), HIS-55::GFP (green), H3K27me2/3 (blue), and H3K36me2 (magenta) in pachytene nuclei. White arrowheads indicate chromosome X identified by the presence of H3 and H3K27me2/3 but absence of H3.3 and H3K36me2. ( C ) Immunofluorescence (Airyscan) of HIS-72::mCherry (red), HIS-55::Ollas (green), and H3K27me2/3 (magenta; top) and H3K36me2 (magenta; bottom) in pachytene nuclei. White arrowheads indicate distinct chromatin domains marked by the colocalization of H3K27me2/3 or H3K36me2 to the presence of H3 or H3.3, respectively. ( D ) Colocalization analysis of Airyscan immunofluorescent micrographs of H3K27me2/3 or H3K36me2 with H3::Ollas and H3.3::mCherry. All quantifications = average ± SE. Unpaired t test; **** P ≤ 0.0001 and ** P ≤ 0.01. Scale bars, 5 μm.

Article Snippet: To quantify the colocalization of tagged histone proteins, H3::Ollas and H3.3::mCherry, with specific PTMs (H3K27me2/3 and H3K36me2) per nuclei, we used the Imaris intensity-based colocalization analysis.

Techniques: Immunofluorescence